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The shape of a galaxys spatially unresolved, globally integrated 21-cm emission line depends on its internal gas kinematics: galaxies with rotation-supported gas disks produce double-horned profiles with steep wings, while galaxies with dispersion-supported gas produce Gaussian-like profiles with sloped wings. Using mock observations of simulated galaxies from the FIRE project, we show that one can therefore constrain a galaxys gas kinematics from its unresolved 21-cm line profile. In particular, we find that the kurtosis of the 21-cm line increases with decreasing $V/sigma$, and that this trend is robust across a wide range of masses, signal-to-noise ratios, and inclinations. We then quantify the shapes of 21-cm line profiles from a morphologically unbiased sample of $sim$2000 low-redshift, HI-detected galaxies with $M_{rm star} = 10^{7-11} M_{odot}$ and compare to the simulated galaxies. At $M_{rm star} gtrsim 10^{10} M_{odot}$, both the observed and simulated galaxies produce double-horned profiles with low kurtosis and steep wings, consistent with rotation-supported disks. Both the observed and simulated line profiles become more Gaussian-like (higher kurtosis and less-steep wings) at lower masses, indicating increased dispersion support. However, the simulated galaxies transition from rotation to dispersion support more strongly: at $M_{rm star} = 10^{8-10}M_{odot}$, most of the simulations produce more Gaussian-like profiles than typical observed galaxies with similar mass, indicating that gas in the low-mass simulated galaxies is, on average, overly dispersion-supported. Most of the lower-mass simulated galaxies also have somewhat lower gas fractions than the median of the observed population. The simulations nevertheless reproduce the observed line-width baryonic Tully-Fisher relation, which is insensitive to rotation vs. dispersion support.
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