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We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off the shelf components, such as a light emitting diode (LED) source, a complementary metal oxide semiconductor (CMOS) image sensor, and a specific combination of optical lenses that allow, through an appropriate software, Fourier transform processing of the sample volume. Waterborne microorganisms, such as Escherichia coli (E. coli), Legionella pneumophila (L. pneumophila) and Phytoplankton, are detected by interrogating the volume sample either in a fluorescent or label-free mode, i.e. with or without fluorescein isothiocyanate (FITC) molecules attached to the micro-organisms, respectively. We achieve a sensitivity of 50 cells/ml, which can be further increased to 0.2 cells/ml by preconcentrating an initial sample volume of 500 ml with an adhoc fluidic system. We also prove the capability of the proposed image cytometer of differentiating microbiological populations by size with a resolution of 3 um and of operating in real contaminated water.
Hierarchical data representations in the context of classi cation and data clustering were put forward during the fties. Recently, hierarchical image representations have gained renewed interest for segmentation purposes. In this paper, we briefly su
Processing of digital images is continuously gaining in volume and relevance, with concomitant demands on data storage, transmission and processing power. Encoding the image information in quantum-mechanical systems instead of classical ones and repl
We propose a method to generate stabilized radio-frequency polarization modulation based on optical frequency combs. Two pulse trains with the same repetition rate and different offset frequencies generate arbitrary polarization states that are modul
In this paper a new formulation of event recognition task is examined: it is required to predict event categories in a gallery of images, for which albums (groups of photos corresponding to a single event) are unknown. We propose the novel two-stage
Motivated by the recent progress in analog computing [Science 343, 160 (2014)], a new approach to perform spatial integration is presented using a dielectric slab waveguide. Our approach is indeed based on the fact that the transmission coefficient o