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Register a new userTransient Optoplasmonic Detection of Single Proteins with Sub-Microsecond Resolution
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Optoplasmonic methods capable of single protein detection so far rely on analyte immobilization in order to facilitate detection [1-6]. These detection schemes, even if they facilitate transient single-molecule detection [7,8] via consequent formation and cleavage of chemical bonds, typically exhibit time resolutions on the order of milliseconds. The need for analyte immobilisation is a direct consequence of the minuscule dimensions of plasmonic near fields typically providing sub-attolitre-sized detection volumes which in turn demand sub-microsecond temporal resolution for the direct detection of proteins in motion. Here we show that such temporal resolution can indeed be achieved. We demonstrate the observation of single proteins as small as Hemoglobin (molecular weight: 64 kDa) as they traverse plasmonic near fields of gold nanorods and interact with their surface, all while maintaining signal-to-noise ratios larger than 5 and an unprecedented temporal resolution well below microseconds. This method enables the label-free observation of single-molecule dynamics on previously unaccessible timescales.
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We report a comparison of two photonic techniques for single-molecule sensing: fluorescence nanoscopy and optoplasmonic sensing. As the test system, oligonucleotides with and without fluorescent labels are transiently hybridized to complementary docking strands attached to gold nanorods. Comparing the measured single-molecule kinetics helps to examine the influence of fluorescent labels as well as factors arising from different sensing geometries. Our results demonstrate that DNA dissociation is not significantly altered by the fluorescent label, while DNA association is affected by geometric factors in the two techniques. These findings open the door to exploiting plasmonic sensing and fluorescence nanoscopy in a complementary fashion, which will aid in building more powerful sensors and uncovering the intricate effects that influence the behavior of single molecules.
For conventional imaging, the imaging resolution limit is given by the Rayleigh criterion. Exploiting the prior knowledge of imaging objects sparsity and fixed optical system, imaging beyond the conventional Rayleigh limit, which is backed up by numerical simulation and experiments, is achieved by illuminating the object with single-shot thermal light and detecting the objects information at the imaging plane with some sparse-array single-pixel detectors. The quality of sub-Rayleigh imaging with sparse detection is also shown to be related to the effective number of single-pixel detectors and the detection signal-to-noise ratio at the imaging plane.
A nanoparticle detection scheme with single particle resolution is presented. The sensor contains only a taper fiber thus offering the advantages of compactness and installation flexibility. Sensing method is based on monitoring the transmitted light power which shows abrupt jumps with each particle binding to the taper surface. The experimental validation of the sensor is demonstrated with polystyrene nanoparticles of radii 120 nm and 175 nm in the 1550 nm wavelength band.
We characterized the S14160-3050HS Multi-Pixel Photon Counter (MPPC), a high efficiency, single channel silicon photomultiplier manufactured by Hamamatsu Photonics K.K. All measurements were performed at a room temperature of (23.0 $pm$ 0.3) $^{circ}$C. We obtained an I-V curve and used relative derivatives to find a breakdown voltage of 38.88 V. At a 3 V over voltage, we find a dark count rate of 1.08 MHz, crosstalk probability of 21 $%$, photon detection efficiency of 55 $%$ at 450 nm, and saturation at 1.0x10$^{11}$ photons per second. The S14160-3050HS MPPC is a candidate detector for the Ultra-Fast Astronomy (UFA) telescope which will characterize the optical (320 nm - 650 nm) sky in the millisecond to sub-microsecond timescales using two photon counting arrays operated in coincidence on the 0.7 meter Nazarbayev University Transient Telescope at the Assy-Turgen Astrophysical Observatory (NUTTelA-TAO) located near Almaty, Kazakhstan. We discuss advantages and disadvantages of using the S14160-3050HS MPPC for the UFA telescope and future ground-based telescopes in sub-second time domain astrophysics.
We propose a new scheme to achieve sub-Rayleigh resolution of interference pattern with independent laser beams. We perform an experimental observation of a double-slit interference with two orthogonally polarized laser beams. The resolution of the interference pattern measured by a two-photon detection is doubled provided the two beams illuminate the double-slit with certain incident angles. The scheme is simple and can be in favor of both high intensity and perfect visibility.
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