Subscribe to the gold package and get unlimited access to Shamra Academy
Register a new userPressure Induced Enlargement and Ionic Current Rectification in Symmetric Nanopores
112
0
0.0
(
0
)
Ask ChatGPT about the research
No Arabic abstract
Nanopores in solid state membranes are a tool able to probe nanofluidic phenomena or can act as a single molecular sensor. They also have diverse applications in filtration, desalination or osmotic power generation. Many of these applications involve chemical, or hydrostatic pressure differences, which act on both the supporting membrane and the ion transport through the pore. By using pressure differences between the sides of the membrane, and an alternating current approach to probe ion transport, we investigate two distinct physical phenomena: the elastic deformation of the membrane through the measurment of strain at the nanopore, and the growth of ionic current rectification with pressure due to pore entrance effects.
rate research
Read More
Nanopores that exhibit ionic current rectification (ICR) behave like diodes, such that they transport ions more efficiently in one direction than the other. Conical nanopores have been shown to rectify ionic current, but only those with at least 500 nm in length exhibit significant ICR. Here, through the finite element method, we show how ICR of conical nanopores with length below 200 nm can be tuned by controlling individual charged surfaces i.e. inner pore surface (surface_inner), and exterior pore surfaces on the tip and base side (surface_tip and surface_base). The charged surface_inner and surface_tip can induce obvious ICR individually, while the effects of the charged surface_base on ICR can be ignored. The fully charged surface_inner alone could render the nanopore counterion-selective and induces significant ion concentration polarization in the tip region, which causes reverse ICR compared to nanopores with all surface charged. In addition, the direction and degree of rectification can be further tuned by the depth of the charged surface_inner. When considering the exterior membrane surface only, the charged surface_tip causes intra-pore ionic enrichment and depletion under opposite biases which results in significant ICR. Its effective region is within ~40 nm beyond the tip orifice. We also found that individual charged parts of the pore system contributed to ICR in an additive way due to the additive effect on the ion concentration regulation along the pore axis. With various combinations of fully/partially charged surface_inner and surface_tip, diverse ICR ratios from ~2 to ~170 can be achieved. Our findings shed light on the mechanism of ionic current rectification in ultra-short conical nanopores, and provide a useful guide to the design and modification of ultra-short conical nanopores in ionic circuits and nanofluidic sensors.
Solid-state nanopores are promising tools for single molecule detection of both DNA and proteins. In this study, we investigate the patterns of ionic current blockades as DNA translocates into or out of the geometric confinement of such conically shaped pores. We studied how the geometry of a nanopore affects the detected ionic current signal of a translocating DNA molecule over a wide range of salt concentration. The blockade level in the ionic current depends on the translocation direction at a high salt concentration, and at lower salt concentrations we find a non-intuitive ionic current decrease and increase within each single event for the DNA translocations exiting from confinement. We use recently developed DNA rulers with markers and finite element calculations to explain our observations. Our calculations explain the shapes of the signals observed at all salt concentrations and show that the unexpected current decrease and increase are due to the competing effects of ion concentration polarization and geometric exclusion of ions. Our analysis shows that over a wide range of geometry, voltage and salt concentrations we are able to understand the ionic current signals of DNA in asymmetric nanopores enabling signal optimization in molecular sensing applications.
Study on a rectified current induced by active particles has received a great attention due to its possible application to a microscopic motor in biological environments. Insertion of an {em asymmetric} passive object amid many active particles has been regarded as an essential ingredient for generating such a rectified motion. Here, we report that the reverse situation is also possible, where the motion of an active object can be rectified by its geometric asymmetry amid many passive particles. This may describe an unidirectional motion of polar biological agents with asymmetric shape. We also find a weak but less diffusive rectified motion in a {em passive} mode without energy pump-in. This moving by dissipation mechanism could be used as a design principle for developing more reliable microscopic motors.
Self-propelled colloidal objects, such as motile bacteria or synthetic microswimmers, have microscopically irreversible individual dynamics - a feature they share with all living systems. The incoherent behaviour of individual swimmers can then be harnessed (or rectified) by microfluidic devices that create systematic motions impossible in equilibrium. Examples include flow of rotor particles round a circuit, steady rotation of a gear wheel in a bacterial bath, and pumping of bacteria between chambers by funnel gates. Here we present a computational proof-of-concept study, showing that such active rectification devices might be created directly from an unstructured primordial soup of motile particles, solely by using spatially modulated illumination to control their local propulsion speed. Alongside both microscopic irreversibility and speed modulation, our mechanism requires spatial symmetry breaking, such as a chevron light pattern, and strong interactions between particles, such as volume exclusion causing a collisional slow-down at high density. These four factors create a many-body rectification mechanism that generically differs from one-body microfluidic antecedents. Our work suggests that standard spatial-light-modulator technology might allow the programmable, light-induced self-assembly of active rectification devices from an unstructured particle bath.
The fluid flow through porous media is described by Darcys law, while the fluid/wall interactions can be neglected. In nanopores, where adsorption dominates, Darcys extension has been made, but approaches able to describe flows in mesopores are still lacking. We show here that molecular flows through nano-constrictions is well described by Berverloos law, predicting the mass flow rate of granular material through macro-apertures. This molecular and granular analogy, allows to derive a relationship providing a theoretical framework for the molecular flow in disordered mesoporous systems.
Log in to be able to interact and post comments
comments
Fetching comments
Sign in to be able to follow your search criteria
