In vivo Study of the effect of seeds extraction of Anise plant in the growth of the two pathogenic fungi Fusarium oxysporum and Aspergillus niger

A Study was carried out to evaluate the ability of the methanolic, hydro and oily extractions of anise seeds (Pimpinella anisum L.) to inhibit the growth of the pathogenic fungi Fusarium oxysporum and Aspergillus niger. The results showed a good inhibition ability of the alcoholic and hydro extractions on the growth of the studied fungi. The inhibition ability differed due to the used concentrations. When the methanolic extraction was used by concentrations (2, 4, and 6) mlg/ ml at the seventh day, the inhibition ability was (42.5, 50.6 and 64.4)% of the fungus F. oxysporum, respectively. And was (23.6, 42.7 and 55.1)% of the fungus A. niger, respectively. Whereas when the hydro extraction was used by the same concentrations, the inhibition ability was (59.2, 65.3 and 70)% respectively for the fungus F. oxysporum, and (46.1, 58 and 61)% for the fungus A. niger, respectively. The oily extraction showed high inhibition ability, where the inhibition rate was 100% when the extraction was used by the two concentrations 50 and 75 μl for both two studied fungi.