A known weight of Abrus seeds powder were soaked in cِhloroformmethanol
(2:1) and extracted for 18 hours, the mixture then filtrated and reextracted
with equal volume of water and evaporated to dryness. The crude
extract is regarded as cِhloroform
-methanol extract of Abrus Seeds (crab's eye).
Chloroform-methanol extract was analyzed by HPLC and GC-MS
chromatography for identification and purification of the extract components.
Trigonelline and Abrine were isolated, a solution of a definite concentration
was made from crab's eye extract and its pure components, trigonelline and
Abrine, then oral administration of this solution of a definite concentration
were given to the Alloxan diabetic rats.
