Carotenoids were separated from the wild type yeast
R.mucilaginosa (A23) and its UV mutant at (254 nm)
R.mucilaginosa (A23-M) using thin layer chromatography
(TLC).The results showed the wild type yeast gave three color
patches, β-Carotene,Torule
ne and Torularhodin with Rf values of
0.9, 0.7 and 0.2 respectively, while the mutant yeast gave only one
spot of color ofTorularhodin at thethe value of Rf = 0.2. Carotenoid
produced by mutant yeast R.mucilaginosa (A23-M) was purified
using a column stocked with polychlorinated Hyflo Super Cel and
magnesium oxide with a ratio of (1: 2). The purified carotenoid was
analyzed by high performance liquid technology chromatography
HPLC at a wavelength 495 nm showing that there was only one
colored compound which was Torularhodin
