A Simple, accurate and highly sensitive spectrophotometric method have been
developed for the rapid determination of Ofloxacine (OFX) in pure form and
pharmaceutical formulations. The visible spectrophotometric method was based on the
formation of
a Orange Yellow colored complex between the (OFX) and 2-amino-6-
nitrobenzothiazole (ANB) at pH 5.2 and 80±5 ºC in 5 min., and λmax at 440 nm. The
effects of analytical parameters on the reported system were investigated. Beer's law was
obeyed in the concentration range of 1.80-86.72 μg/mL, with a percentage relative standard
deviation not exceed ±2.54 and the molar absorptivity coefficients was 3.952×103
(L/mol.cm). Interferences of the other ingredients and excipients were not observed, the
results showed that the developed method with the accuracy and sensitivity highs.
