The purpose of this research was to determine the effect of the corpus luteum in
various stages on morphological characteristics of cumulus oocyte complex consequently
on the yield of morphologically intact oocytes for in vitro culturing.
Oocytes
were collected from cow ovariesof animals after slaughter.
Ovaries were divided into the following four groups: (a) ovaries with corpus
hemorrhagicum; (b) ovaries with a functional corpus luteum;
(c) ovaries with regressing corpus luteum (albicans) ;( d) ovaries without any luteal
structure.
The results of this research show that, the intact oocytes yield for in vitro culturing
differs accordingly with the stage of the corpus luteum.
The highest percentage of intact cell(8.1 ± 1.6) were recorded in ovaries without
corpus luteum , whereas the lowest percentage of intact cells were recorded in ovaries with
regressing corpus luteum(5.5 ± 1.3). There was significant difference (p < 0.001)between
the two groups for intact cells.
