International demand of energy resources increase as
result of economical and social accelerated development,
and under scenarios which predicted depletion of fossil fuel
resource, the efforts trends to alternative energy resource
as biofuel, whe
re a native strain of Botryococcus braunii
was isolated from Room reservoir in Sweida-Syria and
used to produce biofuel, production conditions optimized in
flasks, then turn to work on photobioreactor which made
by native experience. Dry biomass, lipid content of
Botryococcus braunii and optical density were calculated to
express algal growth.