Experimentally detection of genes coding for PSA surface proteins in the genome of Leishmania major strain locally isolated

That study was based on a BLAST search analysis performed against the complete genome sequences using as a query sequence some of the PSA protein sequences reported in the literature. In our study, we have first isolated, cultivated, typed and strain of L. major. Then we have investigated experimentally the complete list of PSA genes coding for membrane proteins in its genome, using PCR approach based on specific primers. In conclusion, we have detected the physical presence of 22 different genes coding for membrane proteins in the genome of Syrian strain of L. major.