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Population III (Pop. III) stars, as of yet, have not been detected, however as we move into the era of extremely large telescopes this is likely to change. One likely tracer for Pop. III stars is the HeII$lambda1640$ emission line, which will be detectable by the HARMONI spectrograph on the European Extremely Large Telescope (ELT) over a broad range of redshifts ($2leq zleq14$). By post-processing galaxies from the cosmological, AMR-hydrodynamical simulation NewHorizon with theoretical spectral energy distributions (SED) for Pop. III stars and radiative transfer (i.e. the Yggdrasil Models and CLOUDY look-up tables respectively) we are able to compute the flux of HeII$lambda1640$ for individual galaxies. From mock 10 hour observations of these galaxies we show that HARMONI will be able to detect Pop. III stars in galaxies up to $zsim10$ provided Pop. III stars have a top heavy Initial Mass Function (IMF). Furthermore, we find that should Pop. III stars instead have an IMF similar to those of the Pop. I stars, the HeII$lambda1640$ line would only be observable for galaxies with Pop. III stellar masses in excess of $10^{7}{,M}_odot,{rm yr}^{-1}$, average stellar age $<1{rm Myr}$ at $z=4$. Finally, we are able to determine the minimal intrinsic flux required for HARMONI to detect Pop. III stars in a galaxy up to $z=10$.
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