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The applications of present nanoscopy techniques for live cell imaging are limited by the long sampling time and low emitter density. Here we developed a new single frame wide-field nanoscopy based on ghost imaging via sparsity constraints (GISC Nanoscopy), in which a spatial random phase modulator is applied in a wide-field microscopy to achieve random measurement for fluorescence signals. This new method can effectively utilize the sparsity of fluorescence emitters to dramatically enhance the imaging resolution to 80 nm by compressive sensing (CS) reconstruction for one raw image. The ultra-high emitter density of 143 {mu}m-2 has been achieved while the precision of single-molecule localization below 25 nm has been maintained. Thereby working with high-density of photo-switchable fluorophores GISC nanoscopy can reduce orders of magnitude sampling frames compared with previous single-molecule localization based super-resolution imaging methods.
The image information acquisition ability of a conventional camera is usually much lower than the Shannon Limit since it does not make use of the correlation between pixels of image data. Applying a random phase modulator to code the spectral images
For remote sensing, high-resolution imaging techniques are helpful to catch more characteristic information of the target. We extend pseudo-thermal light ghost imaging to the area of remote imaging and propose a ghost imaging lidar system. For the fi
Linear super-resolution microscopy via synthesis aperture approach permits fast acquisition because of its wide-field implementations, however, it has been limited in resolution because a missing spatial-frequency band occurs when trying to use a shi
Scattering-type scanning near-field microscopy (s-SNOM) at terahertz (THz) frequencies could become a highly valuable tool for studying a variety of phenomena of both fundamental and applied interest, including mobile carrier excitations or phase tra
Total internal reflection fluorescence microscopy (TIRF) has enabled low-background, live-cell friendly imaging of cell surfaces and other thin samples thanks to the shallow penetration of the evanescent light field into the sample. The implementatio