اشترك بالحزمة الذهبية واحصل على وصول غير محدود شمرا أكاديميا
تسجيل مستخدم جديدActive elastic dimers: Cells moving on rigid tracks
354
0
0.0
(
0
)
اسأل ChatGPT حول البحث
ﻻ يوجد ملخص باللغة العربية
Experiments suggest that the migration of some cells in the three-dimensional extra cellular matrix bears strong resemblance to one-dimensional cell migration. Motivated by this observation, we construct and study a minimal one-dimensional model cell made of two beads and an active spring moving along a rigid track. The active spring models the stress fibers with their myosin-driven contractility and alpha-actinin-driven extendability, while the friction coefficients of the two beads describe the catch/slip bond behavior of the integrins in focal adhesions. In the absence of active noise, net motion arises from an interplay between active contractility (and passive extendability) of the stress fibers and an asymmetry between the front and back of the cell due to catch bond behavior of integrins at the front of the cell and slip bond behavior of integrins at the back. We obtain reasonable cell speeds with independently estimated parameters. We also study the effects of hysteresis in the active spring, due to catch bond behavior and the dynamics of cross-linking, and the addition of active noise on the motion of the cell. Our model highlights the role of alpha-actinin in three-dimensional cell motility and does not require Arp2/3 actin filament nucleation for net motion.
قيم البحث
اقرأ أيضاً
The morphology of an elastic strip subject to vertical compressive stress on a frictional rigid substrate is investigated by a combination of theory and experiment. We find a rich variety of morphologies, which -when the bending elasticity dominates
over the effect of gravity- are classified into three distinct types of states: pinned, partially slipped, and completely slipped, depending on the magnitude of the vertical strain and coefficient of static friction. We develop a theory of elastica under mixed clamped-hinged boundary conditions combined with the Coulomb-Amontons friction law, and find excellent quantitative agreement with simulations and controlled physical experiments. We also discuss the effect of gravity in order to bridge the difference in qualitative behaviors of stiff strips and flexible strings, or ropes. Our study thus complements recent work on elastic rope coiling, and takes a significant step towards establishing a unified understanding of how a thin elastic object interacts vertically with a solid surface.
Cell migration in morphogenesis and cancer metastasis typically involves interplay between different cell types. We construct and study a minimal, one-dimensional model comprised of two different motile cells with each cell represented as an active e
lastic dimer. The interaction between the two cells via cadherins is modeled as a spring that can rupture beyond a threshold force as it undergoes dynamic loading via the attached motile cells. We obtain a phase diagram consisting of chase-and-run dynamics and clumping dynamics as a function of the stiffness of the interaction spring and the threshold force. We also find that while feedback between cadherins and cell-substrate interaction via integrins accentuates the chase-run behavior, feedback is not necessary for it.
The systematic errors due to the practical implementation of the Contact Dynamics method for simulation of dense granular media are examined. It is shown that, using the usual iterative solver to simulate a chain of rigid particles, effective elastic
ity and sound propagation with a finite velocity occur. The characteristics of these phenomena are investigated analytically and numerically in order to assess the limits of applicability of this simulation method and to compare it with soft particle molecular dynamics.
The nonequilibrium activity taking place in a living cell can be monitored with a tracer embedded in the medium. While microrheology experiments based on optical manipulation of such probes have become increasingly standard, we put forward a number o
f experiments with alternative protocols that, we claim, will provide new insight into the energetics of active fluctuations. These are based on either performing thermodynamic--like cycles in control-parameter space, or on determining response to external perturbations of the confining trap beyond simple translation. We illustrate our proposals on an active itinerant Brownian oscillator modeling the dynamics of a probe embedded in a living medium.
We report the synthesis and the temperature dependencies of optical, dielectric, and elastic properties of four homologous dimeric mesogens in the uniaxial nematic (N) phase.
سجل دخول لتتمكن من نشر تعليقات
التعليقات
جاري جلب التعليقات
سجل دخول لتتمكن من متابعة معايير البحث التي قمت باختيارها
