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تسجيل مستخدم جديدNext Generation Epigenetic Detection Technique:Identifying Methylated DNA using Graphene Nanopore
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DNA methylation plays a pivotal role in the genetic evolution of both embryonic and adult cells. For adult somatic cells, location and dynamics of methylation has been very precisely pinned down with the 5-cytosine markers on cytosinephosphate- guanine (CpG) units. Unusual methylation on CpG islands are identified as one of the prime causes for silencing the tumor suppressant genes. Early detection of such methylation can diagnose the potentially harmful oncogenic evolution of cells, and provide a promising guideline for cancer prevention. With this motivation, we propose a cytosine methylation detection technique.Our hypothesis is that electronic signatures of DNA acquired as a molecule translocates through a nanopore, would be significantly different for methylated and non-methylated bases. This difference in electronic fingerprints would allow for reliable real-time differentiations of methylated DNA. We calculate transport currents through a punctured graphene membrane while the cytosine and methylated cytosine translocate through the nanopore. We also calculate the transport properties for uracil and cyanocytosine for comparison. Our calculations of transmission, current, and tunneling conductance show distinct signatures in their spectrum for each molecular type. Thus, in this work, we provide a theoretical analysis that points to a viability of our hypothesis.
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