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We demonstrate a gigapixel inline digital holographic microscopy using a consumer scanner. The consumer scanner can maximally scan an A4 size image (297mm x 210mm) with 4800 dpi (= 5.29 um), theoretically achieving a resolution of 56,144 x 39,698 = 2.22 gigapixels. The system using a consumer scanner has a simple structure, compared with synthetic aperture digital holography using a camera mounted on a two-dimensional moving stage. In this demonstration, we captured an inline hologram with 23,602 x 18,023 pixels (= 0.43 gigapixels). In addition, to accelerate the reconstruction time of the gigapixel hologram and decrease the amount of memory for the reconstruction, we applied the band-limited double-step Fresnel diffraction to the reconstruction.
We report experimental results on heterodyne holographic microscopy of subwavelength-sized gold particles. The apparatus uses continuous green laser illumination of the metal beads in a total internal reflection configuration for dark-field operation
Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light paths in
The use of photonic crystal and negative refractive index materials is known to improve resolution of optical microscopy and lithography devices down to 80 nm level. Here we demonstrate that utilization of well-known digital image recovery techniques
We propose a three-dimensional holographic reconstruction procedure applicable with no a priori knowledge about the recording conditions enabling distortion-free three-dimensional object reconstruction.
We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal spheres radius and refractive index