The evolution of the fractional quantum Hall state at filling 5/2 is studied in density tunable two-dimensional electron systems formed in wide wells in which it is possible to induce a transition from single to two subband occupancy. In 80 and 60 nm
wells, the quantum Hall state at 5/2 filling of the lowest subband is observed even when the second subband is occupied. In a 50 nm well the 5/2 state vanishes upon second subband population. We attribute this distinct behavior to the width dependence of the capacitive energy for intersubband charge transfer and of the overlap of the subband probability densities.
