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The use of structured light beams to detect the velocity of targets moving perpendicularly to the beams propagation axis opens new avenues for remote sensing of moving objects. However, determining the direction of motion is still a challenge since d etection is usually done by means of an interferometric setup which only provides an absolute value of the frequency shift. Here, we put forward a novel method that addresses this issue. It uses dynamic control of the phase in the transverse plane of the structured light beam so that the direction of the particles movement can be deduced. This is done by noting the change in the magnitude of the frequency shift as the transverse phase of the structured light is moved appropriately. We demonstrate our method with rotating micro-particles that are illuminated by a Laguerre-Gaussian beam with a rotating phase about its propagation axis. Our method, which only requires a dynamically configurable optical beam generator, can easily be used with other types of motion by appropriate engineering and dynamic modulation of the phase of the light beam.
We measure the rotational and translational velocity components of particles moving in helical motion using the frequency shift they induced to the structured light beam illuminating them. Under Laguerre-Gaussian mode illumination, a particle with a helical motion reflects light that acquires an additional frequency shift proportional to the angular velocity of rotation in the transverse plane, on top of the usual frequency shift due to the longitudinal motion. We determined both the translational and rotational velocities of the particles by switching between two modes: by illuminating with a Gaussian beam, we can isolate the longitudinal frequency shift; and by using a Laguerre-Gaussian mode, the frequency shift due to the rotation can be determined. Our technique can be used to characterize the motility of microorganisms with a full three-dimensional movement.
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