Accurate estimation of parameters is paramount in developing high-fidelity models for complex dynamical systems. Model-based optimal experiment design (OED) approaches enable systematic design of dynamic experiments to generate input-output data sets
with high information content for parameter estimation. Standard OED approaches however face two challenges: (i) experiment design under incomplete system information due to unknown true parameters, which usually requires many iterations of OED; (ii) incapability of systematically accounting for the inherent uncertainties of complex systems, which can lead to diminished effectiveness of the designed optimal excitation signal as well as violation of system constraints. This paper presents a robust OED approach for nonlinear systems with arbitrarily-shaped time-invariant probabilistic uncertainties. Polynomial chaos is used for efficient uncertainty propagation. The distinct feature of the robust OED approach is the inclusion of chance constraints to ensure constraint satisfaction in a stochastic setting. The presented approach is demonstrated by optimal experimental design for the JAK-STAT5 signaling pathway that regulates various cellular processes in a biological cell.
Ajax applications are designed to have high user interactivity and low user-perceived latency. Real-time dynamic web data such as news headlines, stock tickers, and auction updates need to be propagated to the users as soon as possible. However, Ajax
still suffers from the limitations of the Webs request/response architecture which prevents servers from pushing real-time dynamic web data. Such applications usually use a pull style to obtain the latest updates, where the client actively requests the changes based on a predefined interval. It is possible to overcome this limitation by adopting a push style of interaction where the server broadcasts data when a change occurs on the server side. Both these options have their own trade-offs. This paper explores the fundamental limits of browser-based applications and analyzes push solutions for Ajax technology. It also shows the results of an empirical study comparing push and pull.
