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Terabyte-scale supervised 3D training and benchmarking dataset of the mouse kidney

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 Added by Willy Kuo
 Publication date 2021
and research's language is English




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The performance of machine learning algorithms used for the segmentation of 3D biomedical images lags behind that of the algorithms employed in the classification of 2D photos. This may be explained by the comparative lack of high-volume, high-quality training datasets, which require state-of-the art imaging facilities, domain experts for annotation and large computational and personal resources to create. The HR-Kidney dataset presented in this work bridges this gap by providing 1.7 TB of artefact-corrected synchrotron radiation-based X-ray phase-contrast microtomography images of whole mouse kidneys and validated segmentations of 33 729 glomeruli, which represents a 1-2 orders of magnitude increase over currently available biomedical datasets. The dataset further contains the underlying raw data, classical segmentations of renal vasculature and uriniferous tubules, as well as true 3D manual annotations. By removing limits currently imposed by small training datasets, the provided data open up the possibility for disruptions in machine learning for biomedical image analysis.

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The hearts, kidneys, livers, spleens and brains of ${}^57$Fe enriched wild-type and heterozygous $beta$-thalassaemic mice at 1, 3, 6 and 9 months of age were studied by means of Mossbauer Spectroscopy at 80K. Ferritin-like iron depositions in the heart and the brain of the thalassaemic mice were found to be slightly increased while significant amounts of Ferritin-like iron were found in the kidneys, liver and spleen. The ferritin-like iron doublet, found in the organs, could be further separated into two sub-doublets representing the inner and surface structures of ferritin mineral core. Surface iron sites were found to be predominant in the hearts and brains of all mice and in the kidneys of the wild-type animals. Ferritin rich in inner iron sites was predominant in the kidneys of the thalassaemic mice, as well as in the livers and in the spleens. The inner-to-surface iron sites ratio was elevated in all thalassaemic samples indicating that besides ferritin amount, the disease can also affect ferritin mineral core structure.
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