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Register a new userA State-of-the-art Survey of Object Detection Techniques in Microorganism Image Analysis: from Traditional Image Processing and Classical Machine Learning to Current Deep Convolutional Neural Networks and Potential Visual Transformers
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Pingli Ma
Publication date
2021
fields
Informatics Engineering
and research's language is
English
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Microorganisms play a vital role in human life. Therefore, microorganism detection is of great significance to human beings. However, the traditional manual microscopic detection methods have the disadvantages of long detection cycle, low detection accuracy in large orders, and great difficulty in detecting uncommon microorganisms. Therefore, it is meaningful to apply computer image analysis technology to the field of microorganism detection. Computer image analysis can realize high-precision and high-efficiency detection of microorganisms. In this review, first,we analyse the existing microorganism detection methods in chronological order, from traditional image processing and traditional machine learning to deep learning methods. Then, we analyze and summarize these existing methods and introduce some potential methods, including visual transformers. In the end, the future development direction and challenges of microorganism detection are discussed. In general, we have summarized 137 related technical papers from 1985 to the present. This review will help researchers have a more comprehensive understanding of the development process, research status, and future trends in the field of microorganism detection and provide a reference for researchers in other fields.
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Microorganisms such as bacteria and fungi play essential roles in many application fields, like biotechnique, medical technique and industrial domain. Microorganism counting techniques are crucial in microorganism analysis, helping biologists and related researchers quantitatively analyze the microorganisms and calculate their characteristics, such as biomass concentration and biological activity. However, traditional microorganism manual counting methods, such as plate counting method, hemocytometry and turbidimetry, are time-consuming, subjective and need complex operations, which are difficult to be applied in large-scale applications. In order to improve this situation, image analysis is applied for microorganism counting since the 1980s, which consists of digital image processing, image segmentation, image classification and suchlike. Image analysis-based microorganism counting methods are efficient comparing with traditional plate counting methods. In this article, we have studied the development of microorganism counting methods using digital image analysis. Firstly, the microorganisms are grouped as bacteria and other microorganisms. Then, the related articles are summarized based on image segmentation methods. Each part of the article is reviewed by methodologies. Moreover, commonly used image processing methods for microorganism counting are summarized and analyzed to find common technological points. More than 144 papers are outlined in this article. In conclusion, this paper provides new ideas for the future development trend of microorganism counting, and provides systematic suggestions for implementing integrated microorganism counting systems in the future. Researchers in other fields can refer to the techniques analyzed in this paper.
In recent years, deep learning has made brilliant achievements in image classification. However, image classification of small datasets is still not obtained good research results. This article first briefly explains the application and characteristics of convolutional neural networks and visual transformers. Meanwhile, the influence of small data set on classification and the solution are introduced. Then a series of experiments are carried out on the small datasets by using various models, and the problems of some models in the experiments are discussed. Through the comparison of experimental results, the recommended deep learning model is given according to the model application environment. Finally, we give directions for future work.
Nowadays, analysis of Transparent Environmental Microorganism Images (T-EM images) in the field of computer vision has gradually become a new and interesting spot. This paper compares different deep learning classification performance for the problem that T-EM images are challenging to analyze. We crop the T-EM images into 8 * 8 and 224 * 224 pixel patches in the same proportion and then divide the two different pixel patches into foreground and background according to ground truth. We also use four convolutional neural networks and a novel ViT network model to compare the foreground and background classification experiments. We conclude that ViT performs the worst in classifying 8 * 8 pixel patches, but it outperforms most convolutional neural networks in classifying 224 * 224 pixel patches.
Cervical cancer is a very common and fatal cancer in women. Cytopathology images are often used to screen this cancer. Since there is a possibility of a large number of errors in manual screening, the computer-aided diagnosis system based on deep learning is developed. The deep learning methods required a fixed size of input images, but the sizes of the clinical medical images are inconsistent. The internal cell ratios of the images are suffered while resizing it directly. Clinically, the ratios of cells inside cytopathological images provide important information for doctors to diagnose cancer. Therefore, it is illogical to resize directly. However, many existing studies resized the images directly and obtained very robust classification results. To find a reasonable interpretation, we have conducted a series of comparative experiments. First, the raw data of the SIPaKMeD dataset are preprocessed to obtain the standard and scaled datasets. Then, the datasets are resized to 224 $times$ 224 pixels. Finally, twenty-two deep learning models are used to classify standard and scaled datasets. The conclusion is that the deep learning models are robust to changes in the internal cell ratio of cervical cytopathological images. This conclusion is also validated on the Herlev dataset.
Image forensic plays a crucial role in both criminal investigations (e.g., dissemination of fake images to spread racial hate or false narratives about specific ethnicity groups) and civil litigation (e.g., defamation). Increasingly, machine learning approaches are also utilized in image forensics. However, there are also a number of limitations and vulnerabilities associated with machine learning-based approaches, for example how to detect adversarial (image) examples, with real-world consequences (e.g., inadmissible evidence, or wrongful conviction). Therefore, with a focus on image forensics, this paper surveys techniques that can be used to enhance the robustness of machine learning-based binary manipulation detectors in various adversarial scenarios.
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