Subscribe to the gold package and get unlimited access to Shamra Academy
Register a new userStructural evolution of protein-biofilms: Simulations and Experiments
130
0
0.0
(
0
)
Authors
Y. Schmitt
Ask ChatGPT about the research
No Arabic abstract
The control of biofilm formation is a challenging goal that has not been reached yet in many aspects. One is the role of van der Waals forces and another the importance of mutual interactions between the adsorbing and the adsorbed biomolecules (critical crowding). Here, a combined exeperimental and theoretical approach is presented that fundamentally probes both aspects. On three model proteins, lysozyme, {alpha}-amylase and bovine serum albumin (BSA), the adsorption kinetics is studied. Composite substrates are used enabling a separation of the short- and the long-range forces. Though usually neglected, experimental evidence is given for the influence of van der Waals forces on the protein adsorption as revealed by in situ ellipsometry. The three proteins were chosen for their different conformational stability in order to investigate the influence of conformational changes on the adsorption kinetics. Monte Carlo simulations are used to develop a model for these experimental results by assuming an internal degree of freedom to represent conformational changes. The simulations also provide data on the distribution of adsorption sites. By in situ atomic force microscopy we can also test this distribution experimentally which opens the possibility to e.g. investigate the interactions between adsorbed proteins.
rate research
Read More
While a significant body of investigations have been focused on the process of protein self-assembly, much less is understood about the reverse process of a filament breaking due to thermal motion into smaller fragments, or depolymerization of subunits from the filament ends. Indirect evidence for actin and amyloid filament fragmentation has been reported, although the phenomenon has never been directly observed either experimentally or in simulations. Here we report the direct observation of filament depolymerization and breakup in a minimal, calibrated model of coarse-grained molecular simulation. We quantify the orders of magnitude by which the depolymerization rate from the filament ends $k_mathrm{off}$ is larger than fragmentation rate $k_{-}$ and establish the law $k_mathrm{off}/k_- = exp [( varepsilon_| - varepsilon_bot) / k_mathrm{B}T ] = exp [0.5 varepsilon / k_mathrm{B}T ]$, which accounts for the topology and energy of bonds holding the filament together. This mechanism and the order-of-magnitude predictions are well supported by direct experimental measurements of depolymerization of insulin amyloid filaments.
We present a large-scale numerical study, supplemented by experimental observations, of a quasi-two-dimensional active system of polar rods and spherical beads confined between two horizontal plates and energised by vertical vibration. For low rod concentrations $Phi_r$ we observe a direct phase transition, as bead concentration $Phi_b$ is increased, from the isotropic phase to a homogeneous flock. For $Phi_r$ above a threshold value, an ordered band dense in both rods and beads occurs between the disordered phase and the homogeneous flock, in both experiments and simulations. Within the size ranges accessible we observe only a single band, whose width increases with $Phi_r$. Deep in the ordered state, we observe broken-symmetry sound modes and giant number fluctuations. The direction-dependent sound speeds and the scaling of fluctuations are consistent with the predictions of field theories of flocking, but sound damping rates show departures from such theories. At very high densities we see phase separation into rod-rich and bead-rich regions, both of which move coherently.
Surface freezing is a phenomenon in which crystallization is enhanced at a vapor-liquid interface. In some systems, such as $n$-alkanes, this enhancement is dramatic, and results in the formation of a crystalline layer at the free interface even at temperatures slightly above the equilibrium bulk freezing temperature. There are, however, systems in which the enhancement is purely kinetic, and only involves faster nucleation at or near the interface. The first, thermodynamic, type of surface freezing is easier to confirm in experiments, requiring only the verification of the existence of crystalline order at the interface. The second, kinetic, type of surface freezing is far more difficult to prove experimentally. One material that is suspected of undergoing the second type of surface freezing is liquid water. Despite strong indications that the freezing of liquid water is kinetically enhanced at vapor-liquid interfaces, the findings are far from conclusive, and the topic remains controversial. In this perspective, we present a simple thermodynamic framework to understand conceptually and distinguish these two types of surface freezing. We then briefly survey fifteen years of experimental and computational work aimed at elucidating the surface freezing conundrum in water.
Bacterial biofilms, surface-attached communities of cells, are in some respects similar to colloidal solids; both are densely packed with non-zero yield stresses. However, unlike non-living materials, bacteria reproduce and die, breaking mechanical equilibrium and inducing collective dynamic responses. We report experiments and theory investigating the motion of immotile Vibrio cholerae, which can kill each other and reproduce in biofilms. We vary viscosity by using bacterial variants that secrete different amounts of extracellular matrix polymers, but are otherwise identical. Unlike thermally-driven diffusion, in which diffusivity decreases with increased viscosity, we find that cellular motion mediated by death and reproduction is independent of viscosity over timescales relevant to bacterial reproduction. To understand this surprising result, we use two separate modeling approaches. First we perform explicitly mechanical simulations of one-dimensional chains of Voigt-Kelvin elements that can die and reproduce. Next, we perform an independent statistical approach, modeling Brownian motion with the classic Langevin equation under an effective temperature that depends on cellular division rate. The diffusion of cells in both approaches agrees quite well, supporting a kinetic interpretation for the effective temperature used here and developed in previous work. As the viscoelastic behavior of biofilms is believed to play a large role in their anomalous biological properties, such as antibiotic resistance, the independence of cellular diffusive motion --- important for biofilm growth and remodeling --- on viscoelastic properties likely holds ecological, medical, and industrial relevance.
Water plays a fundamental role in protein stability. However, the effect of the properties of water on the behaviour of proteins is only partially understood. Several theories have been proposed to give insight into the mechanisms of cold and pressure denaturation, or the limits of temperature and pressure above which no protein has a stable, functional state, or how unfolding and aggregation are related. Here we review our results based on a theoretical approach that can rationalise the water contribution to protein solutions free energy. We show, using Monte Carlo simulations, how we can rationalise experimental data with our recent results. We discuss how our findings can help develop new strategies for the design of novel synthetic biopolymers or possible approaches for mitigating neurodegenerative pathologies.
Log in to be able to interact and post comments
comments
Fetching comments
Sign in to be able to follow your search criteria
