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Register a new userLocal Analysis of Heterogeneous Intracellular Transport: Slow and Fast moving Endosomes
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Trajectories of endosomes inside living eukaryotic cells are highly heterogeneous in space and time and diffuse anomalously due to a combination of viscoelasticity, caging, aggregation and active transport. Some of the trajectories display switching between persistent and anti-persistent motion while others jiggle around in one position for the whole measurement time. By splitting the ensemble of endosome trajectories into slow moving sub-diffusive and fast moving super-diffusive endosomes, we analyzed them separately. The mean squared displacements and velocity auto-correlation functions confirm the effectiveness of the splitting methods. Applying the local analysis, we show that both ensembles are characterized by a spectrum of local anomalous exponents and local generalized diffusion coefficients. Slow and fast endsomes have exponential distributions of local anomalous exponents and power law distributions of generalized diffusion coefficients. This suggests that heterogeneous fractional Brownian motion is an appropriate model for both fast and slow moving endosomes. This article is part of a Special Issue entitled: Recent Advances In Single-Particle Tracking: Experiment and Analysis edited by Janusz Szwabinski and Aleksander Weron.
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A major open problem in biophysics is to understand the highly heterogeneous transport of many structures inside living cells, such as endosomes. We find that mathematically it is described by spatio-temporal heterogeneous fractional Brownian motion (hFBM) which is defined as FBM with a randomly switching anomalous exponent and random generalized diffusion coefficient. Using a comprehensive local analysis of a large ensemble of experimental endosome trajectories (> 10^5), we show that their motion is characterized by power-law probability distributions of displacements and displacement increments, exponential probability distributions of local anomalous exponents and power-law probability distributions of local generalized diffusion coefficients of endosomes which are crucial ingredients of spatio-temporal hFBM. The increased sensitivity of deep learning neural networks for FBM characterisation corroborates the development of this multi-fractal analysis. Our findings are an important step in understanding endosome transport. We also provide a powerful tool for studying other heterogeneous cellular processes.
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