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Register a new userStudies of unicellular micro-organisms Saccharomyces cerevisiae by means of Positron Annihilation Lifetime Spectroscopy
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Results of Positron Annihilation Lifetime Spectroscopy (PALS) and microscopic studies on simple microorganisms: brewing yeasts are presented. Lifetime of ortho - positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer lived component) for lyophilised and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water - the main component of the cell - affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilised, dried and wet yeasts with best possible resolution were obtained using Inverted Microscopy (IM) and Environmental Scanning Electron Microscopy (ESEM) methods. As a result visible changes to the surface of the cell membrane were observed in ESEM images.
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Sub-micron defects represent a well-known fundamental problem in manufacturing since they can significantly affect performance and lifetime of virtually any high-value component. Positron annihilation lifetime spectroscopy is arguably the only established method capable of detecting defects down to the sub-nanometer scale but, to date, it only works for surface studies, and with limited resolution. Here, we experimentally and numerically show that laser-driven systems can overcome these well-known limitations, by generating ultra-short positron beams with a kinetic energy tuneable from 500 keV up to 2 MeV and a number of positrons per shot in a 50 keV energy slice color{black} of the order of $10^3$. Numerical simulations of the expected performance of a typical mJ-scale kHz laser demonstrate the possibility of generating MeV-scale narrow-band and ultra-short positron beams with a flux exceeding $10^5$ positrons/s, of interest for fast volumetric scanning of materials at high resolution.
This paper develops mathematical models describing the evolutionary dynamics of both asexually and sexually reproducing populations of diploid unicellular organisms. We consider two forms of genome organization. In one case, we assume that the genome consists of two multi-gene chromosomes, while in the second case we assume that each gene defines a separate chromosome. If the organism has $ l $ homologous pairs that lack a functional copy of the given gene, then the fitness of the organism is $ kappa_l $. The $ kappa_l $ are assumed to be monotonically decreasing, so that $ kappa_0 = 1 > kappa_1 > kappa_2 > ... > kappa_{infty} = 0 $. For nearly all of the reproduction strategies we consider, we find, in the limit of large $ N $, that the mean fitness at mutation-selection balance is $ max{2 e^{-mu} - 1, 0} $, where $ N $ is the number of genes in the haploid set of the genome, $ epsilon $ is the probability that a given DNA template strand of a given gene produces a mutated daughter during replication, and $ mu = N epsilon $. The only exception is the sexual reproduction pathway for the multi-chromosomed genome. Assuming a multiplicative fitness landscape where $ kappa_l = alpha^{l} $ for $ alpha in (0, 1) $, this strategy is found to have a mean fitness that exceeds the mean fitness of all of the other strategies. Furthermore, while the other reproduction strategies experience a total loss of viability due to the steady accumulation of deleterious mutations once $ mu $ exceeds $ ln 2 $, no such transition occurs in the sexual pathway. The results of this paper demonstrate a selective advantage for sexual reproduction with fewer and much less restrictive assumptions than previous work.
The Jagiellonian Positron Emission Tomograph (J-PET) is the first PET device built from plastic scintillators. It is a multi-purpose detector designed for medical imaging and for studies of properties of positronium atoms in porous matter and in living organisms. In this article we report on the commissioning of the J-PET detector in view of studies of positronium decays. We present results of analysis of the positron lifetime measured in the porous polymer. The obtained results prove that J-PET is capable of performing simultaneous imaging of the density distribution of annihilation points as well as positron annihilation lifetime spectroscopy.
The muon collider represents one of the most promising solutions for a future machine exploring the high energy frontier, but several challenges due to the 2.2 $mu$sec muon lifetime at rest have to be carefully considered. The LEMMA project is investigating the possibility of producing low emittance muon/antimuon pairs from the e$^+$e$^-$ annihilation process at threshold energy, resulting in small transverse emittance beams without any additional beam cooling. However most of the measurements available are performed at higher $sqrt{s}$ values. It is therefore necessary to measure muons production in positron annihilation at threshold energy and compare the experimental results with the predictions in this specific energy regime. Apart from being a topic of physical interest by itself, these near to threshold measurements can have a sizeable impact on the estimation of the ultimate luminosity achievable in a muon collider with the LEMMA injection scheme.
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