That study was based on a BLAST search analysis performed against the
complete genome sequences using as a query sequence some of the PSA protein
sequences reported in the literature. In our study, we have first isolated,
cultivated, typed and str
ain of L. major. Then we have investigated
experimentally the complete list of PSA genes coding for membrane proteins in
its genome, using PCR approach based on specific primers. In conclusion, we
have detected the physical presence of 22 different genes coding for membrane
proteins in the genome of Syrian strain of L. major.