Sublethal toxicity studies were conducted with a rotifera (Brachionus
calyciflorus) to determine the No observed effect concentrations (NOECs) of a
different eight toxic substances based on the protein mass reduction. Protein
mass (with moleculer
weight 43KDa - 67KDa) was quantified using
electrophoresis method. Quantitation of protein concentrations demonstrated
that toxicant stress reduced protein concentrations. The NOECs-43KDa and
NOECs - 67KDa were reported. Since in vivo protein cocentrations can be
assessed in a short time, these results can be obtained in only a few hours,
which is substantially less than traditional whole animal reproductive tests.
NOECs - NOECs - 43KDa test were generally more sensitive end points than
NOECs - NOECs-67KDa test and more sensitive than standard whole animal
toxicity tests for only four toxicants.
