Study of semileptonic decays of neutral kaons allows to perform a test of discrete symmetries, as well as basic principles of the Standard Model. In this paper a general review on dependency between charge asymmetry constructed for semileptonic decays of short- and long-lived kaons and CPT symmetry is given. The current status of determination of charge asymmetry for short-lived kaon, obtained by reconstruction of about 10^5 KS -> pen decays collected at DAFNE with the KLOE detector is also reviewed.