We report a new measurement of the $n=2$ Lamb shift in Muonium using microwave spectroscopy. Our result of $1047.2(2.3)_textrm{stat}(1.1)_textrm{syst}$ MHz comprises an order of magnitude improvement upon the previous best measurement. This value matches the theoretical calculation within one standard deviation allowing us to set limits on CPT violation in the muonic sector, as well as on new physics coupled to muons and electrons which could provide an explanation of the muon $g-2$ anomaly.
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