We compute the quantum cohomology of symplectic flag manifolds. Symplectic flag manifolds can be described by non-abelian GLSMs with superpotential. Although the ring relations cannot be directly read off from the equations of motion on the Coulomb branch due to complication introduced by the non-abelian gauge symmetry, it can be shown that they can be extracted from the localization formula in a gauge-invariant form. Our result is general for all symplectic flag manifolds, which reduces to previously established results on symplectic Grassmannians and complete symplectic flag manifolds derived by other means. We also explain why a (0,2) deformation of the GLSM does not give rise to a deformation of the quantum cohomology.