We present the low-resolution transmission spectra of the puffy hot Jupiter HAT-P-65b (0.53 M$_mathrm{Jup}$, 1.89 R$_mathrm{Jup}$, $T_mathrm{eq}=1930$ K), based on two transits observed using the OSIRIS spectrograph on the 10.4 m Gran Telescopio CANARIAS (GTC). The transmission spectra of the two nights are consistent, covering the wavelength range 517--938 nm and consisting of mostly 5 nm spectral bins. We perform equilibrium-chemistry spectral retrieval analyses on the jointly fitted transmission spectrum and obtain an equilibrium temperature of $1645^{+255}_{-244}$ K and a cloud coverage of $36^{+23}_{-17}$%, revealing a relatively clear planetary atmosphere. Based on free-chemistry retrieval, we report strong evidence for TiO. Additional individual analyses in each night reveal weak-to-moderate evidence for TiO in both nights, but moderate evidence for Na or VO only in one of the nights. Future high-resolution Doppler spectroscopy as well as emission observations will help confirm the presence of TiO and constrain its role in shaping the vertical thermal structure of HAT-P-65bs atmosphere.
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