Grand unification groups (GUTs) are constructed from SO(32) heterotic string via $Z_{12-I}$ orbifold compactification. So far, most phenomenological studies from string compactification relied on $EE8$ heterotic string, and this invites the SO(32) heterotic string very useful for future phenomenological studies. Here, spontaneous symmetry breaking is achieved by Higgsing of the anti-symmetric tensor representations of SU($N$). The anti-SU($N$) presented in this paper is a completely different class from the flipped-SU($N$)s from the spinor representations of SO($2N$). Here, we realize chiral representations: $tsixoplus 5cdot ineb $ for a SU(9) GUT and $3{{ten}_Loplus {fiveb}_L}$ for a SU(5)$$ GUT. In particular, we confirm that the non-Abelian anomalies of SU(9) gauge group vanish and hence our compactification scheme achieves the key requirement. We also present the Yukawa couplings, in particular for the heaviest fermion, $t$, and lightest fermions, neutrinos. In the supersymmetric version, we present a scenario how supersymmetry can be broken dynamically via the confining gauge group SU(9). Three families in the visible sector are interpreted as the chiral spectra of SU(5)$$ GUT.