In light of the recent LHC Higgs data, we examine the parameter space of type II two-Higgs-doublet model in which the 125 GeV Higgs has the wrong sign Yukawa couplings. Combining related theoretical and experimental limits, we find that the LHC Higgs data exclude most of the parameter space of the wrong sign Yukawa coupling. For $m_H=$ 600 GeV, the allowed samples are mainly distributed in several corners and narrow bands of m_A<20 GeV, 30 GeV<m_A<120 GeV, 240 GeV<m_A<300 GeV, 380 GeV <m_A<430 GeV, and 480 GeV<m_A<550 GeV. For m_A=600 GeV, m_H is required to be less than 470 GeV. The light pseudo-scalar with a mass of 20 GeV is still allowed in case of the wrong sign Yukawa coupling of 125 GeV Higgs.
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