We find a novel mechanism for generating transverse single-spin asymmetry (SSA) in semi-inclusive deep inelastic scattering, distinct from the known ones which involve the Sivers and Collins functions, or their collinear twist-three counterparts. It is demonstrated that a phase needed for SSA can be produced purely within a parton-level cross section starting at two loops. We identify the complete set of two-loop diagrams for SSA, and discuss their gauge invariance and collinear factorization which features the $g_T$ distribution function. In the $k_T$ factorization framework, many more sources for SSA exist, and contributions from all possible two-parton transverse-momentum-dependent parton distribution functions are presented up to two loops and twist three.
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