Using the sample produced by the AMPT default model, we construct a corresponding mixed sample by the method of mixed events. The mixed sample provides an effective estimation for non-critical fluctuations which are caused by global and systematic effects. The dynamical cumulants of conserved charges are defined as the cumulants of the original sample minus the cumulants of the mixed sample. It is demonstrated that dynamical cumulants are subtracted statistical fluctuations, and centrality bin width or detection efficiency independent, in consistent with formulae corrected cumulants. Therefore, dynamical cumulants are helpful in obtaining critical fluctuations at the RHIC BES.