We derive the complete set of evolutions of chirality-odd twist-3 fragmentation functions at one-loop level. There are totally nine real twist-3 fragmentation functions, among which seven are independent. The renormalization-scale dependence of the nine functions has an important implication for studies of single transverse-spin asymmetries. We find that the evolutions of the three complex fragmentation functions defined by quark-gluon-quark operator are mixed with themselves. There is no mixing with the fragmentation functions defined only with bilinear quark field operators. In the large-$N_c$ limit the evolutions of the three complex fragmentation functions are simplified and reduced to six homogenous equations.
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