The measurement of the ionization produced by particles in a medium presents a great interest in several fields from metrology to particule physics and cosmology. The ionization quenching factor is defined as the fraction of energy released by ionisation by a recoil in a medium compared with its kinetic energy. At low energy, in the range of a few keV, the ionization falls rapidly and systematic measurement are needed. We have developped an experimental setup devoted to the measurement of low energy (keV) ionization quenching factor for the MIMAC project. The ionization produced in the gas has been measured with a Micromegas detector filled with Helium gas mixture.