Using a two-channel model, we show that the number of closed channel molecules in a two-component Fermi gas close to a Feshbach resonance is directly related to the derivative of the energy of the gas with respect to the inverse scattering length. We extract this quantity from the fixed node Monte-Carlo equation of state and we compare to the number of closed channel molecules measured in the Rice experiment with lithium [Partridge et al., Phys. Rev. Lett. 95, 020404 (2005)]. We also discuss the effect of a difference between the trapping potentials seen by a closed-channel molecule and by an open-channel pair of atoms in terms of an effective position-dependent scattering length.
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