We extend the virial equation of state to include 3H and 3He nuclei, and predict significant mass-three fractions near the neutrinosphere in supernovae. While alpha particles are often more abundant, we demonstrate that energy transfer cross-sections for muon and tau neutrinos at low densities are dominated by breakup of the loosely-bound 3H and 3He nuclei. The virial coefficients involving A=3 nuclei are calculated directly from the corresponding nucleon-3H and nucleon-3He scattering phase shifts. For the neutral-current inelastic cross-sections and the energy transfer cross sections, we perform ab-initio calculations based on microscopic two- and three-nucleon interactions and meson-exchange currents.