We present an improved determination of the CP and CPT violation parameters Re(epsilon) and Im(delta) based on the unitarity condition (Bell-Steinberger relation) and on recent results from the KLOE experiment. We find Re(epsilon) = (159.6 pm 1.3)10^-5 and Im(delta) = (0.4 pm 2.1)10^-5, consistent with no CPT violation.