From muon spin rotation measurements on under- to overdoped Bi-2212 crystals we obtain evidence for a two-stage transition of the vortex matter as a function of temperature. The first transition is well known and related to the irreversibility line (IL). The second one is located below the IL and has not been previously observed. It occurs for all three sets of crystals and is unrelated to the vortex mobility. Our data are consistent with a two-stage melting scenario where the intra-planar melting of the vortex lattice and the inter-planar decoupling of the vortex lines occur independently.
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