We report the field-orientation dependent specific heat of the spin-triplet superconductor Sr2RuO4 under the magnetic field aligned parallel to the RuO2 planes with high accuracy. Below about 0.3 K, striking 4-fold oscillations of the density of states reflecting the superconducting gap structure have been resolved for the first time. We also obtained strong evidence of multi-band superconductivity and concluded that the superconducting gap in the active band, responsible for the superconducting instability, is modulated with a minimum along the [100] direction.