We have investigated the relationship between the 2-10 keV X-ray variability amplitude and black hole mass for a sample of 46 radio-quiet active galactic nuclei observed by ASCA. Thirty-three of the objects in our sample exhibited variability over a time-scale of ~40 ks, and we found a significant anti-correlation between excess variance and mass. Unlike most previous studies, we have quantified the variability using nearly the same time-scale for all objects. Moreover, we provide a prescription for estimating the uncertainties in excess variance which accounts both for measurement uncertainties and for the stochastic nature of the variability. We also present an analytical method to predict the excess variance from a model power spectrum accounting for binning, sampling and windowing effects. Using this, we modelled the variance-mass relation assuming all objects have a universal twice-broken power spectrum, with the position of the breaks being dependent on mass. This accounts for the general form of the relationship but there is considerable scatter. We investigated this scatter as a function of the X-ray photon index, luminosity and Eddington ratio. After accounting for the dependence of excess variance on mass, we find no significant correlation with either luminosity or X-ray spectral slope. We do find an anti-correlation between excess variance and the Eddington ratio, although this relation might be an artifact owing to the uncertainties in the mass measurements. It remains to be established that enhanced X-ray variability is a property of objects with steep X-ray slopes or large Eddington ratios.
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