A C-symplectic structure is a complex-valued 2-form which is holomorphically symplectic for an appropriate complex structure. We prove an analogue of Mosers isotopy theorem for families of C-symplectic structures and list several applications of this result. We prove that the degenerate twistorial deformation associated to a holomorphic Lagrangian fibration is locally trivial over the base of this fibration. This is used to extend several theorems about Lagrangian fibrations, known for projective hyperkahler manifolds, to the non-projective case. We also exhibit new examples of non-compact complex manifolds with infinitely many pairwise non-birational algebraic compactifications.