In this paper, we study the uniqueness of zero-order entire functions and their difference. We have proved: Let $f(z)$ be a nonconstant entire function of zero order, let $q eq0, eta$ be two finite complex numbers, and let $a$ and $b$ be two distinct complex numbers. If $f(z)$ and $Delta_{q,eta}f(z)$ share $a$, $b$ IM, then $fequiv Delta_{q,eta}f$.