We propose a new type of instanton interference effect in two-dimensional higher-order topological insulators. The intercorner tunneling consists of the instanton and the anti-instanton pairs that travel through the boundary of the higher-order topological insulator. The Berry phase difference between the instanton pairs causes the interference of the tunneling. This topological effect leads to the gate-tunable oscillation of the energy splitting between the corner states, where the oscillatory nodes signal the perfect suppression of the tunneling. We suggest this phenomenon as a unique feature of the topological corner states that differentiate from trivial bound states. In the view of experimental realization, we exemplify twisted bilayer graphene, as a promising candidate of a two-dimensional higher-order topological insulator. The oscillation can be readily observed through the transport experiment that we propose. Thus, our work provides a feasible route to identify higher-order topological materials.
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