We report the fabrication of a low cost, and highly reproducible large scale surface-enhanced Raman spectroscopy substrate using an inkjet-printed Ag nanoparticle ink (AgNI). The AgNI SERS substrates were evaluated for SERS using BPY as a molecular probe. The printed AgNI dot arrays exhibit an excellent SERS performance and reproducibility. The batch to batch and spot to spot standard deviation value of less than 10 percent was obtained. The results reveal the reproducibility of the AgNI SERS dot arrays and its potential application for SERS substrates.