Let $D=(G,mathcal{O},w)$ be a weighted oriented graph whose edge ideal is $I(D)$. In this paper, we characterize the unmixed property of $I(D)$ for each one of the following cases: $G$ is an $SCQ$ graph; $G$ is a chordal graph; $G$ is a simplicial graph; $G$ is a perfect graph; $G$ has no $4$- or $5$-cycles; $G$ is a graph without $3$- and $5$-cycles; and ${rm girth}(G)geqslant 5$.
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