This volume is a collection of contributions for the 7-week program Probing Nucleons and Nuclei in High Energy Collisions that was held at the Institute for Nuclear Theory in Seattle, WA, USA, from October 1 until November 16, 2018. The program was dedicated to the physics of the Electron Ion Collider (EIC), the worlds first polarized electron-nucleon (ep) and electron-nucleus (eA) collider to be constructed in the USA. These proceedings are organized by chapters, corresponding to the weeks of the program: Week I, Generalized parton distributions; Week II, Transverse spin and TMDs; Week III, Longitudinal spin; Week IV, Symposium week; Weeks V & VI, eA collisions; Week VII, pA and AA collisions. We hope these proceedings will be useful to readers as a compilation of EIC-related science at the end of the second decade of the XXI century.
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