We give an explicit description of the irreducible components of two-row Springer fibers in type A as closed subvarieties in certain Nakajima quiver varieties in terms of quiver representations. By taking invariants under a variety automorphism, we obtain an explicit algebraic description of the irreducible components of two-row Springer fibers of classical type. As a consequence, we discover relations on isotropic flags that describe the irreducible components.