In this paper, we are concerned with regularity of suitable weak solutions of the 3D Navier-Stokes equations in Lorentz spaces. We obtain $varepsilon$-regularity criteria in terms of either the velocity, the gradient of the velocity, the vorticity, or deformation tensor in Lorentz spaces. As an application, this allows us to extend the result involving Lerays blow up rate in time, and to show that the number of singular points of weak solutions belonging to $ L^{p,infty}(-1,0;L^{q,l}(mathbb{R}^{3})) $ and $ {2}/{p}+{3}/{q}=1$ with $3<q<infty$ and $qleq l <infty$ is finite.